If you intend to use your plant growth chambers or rooms for tissue culture research, it is important to note that your chamber may not be well suited for tissue culture experiments in petri dishes. Should you place petri dishes in your plant growth chamber or room that features either a horizontal or downward airflow design - you may experience condensation on the underside of the lid of the petri dish. Below is a photograph of a plant growth chamber with horizontal airflow that is improperly being used for tissue culture research:
As indicated in the above image, condensation is forming on the underside of each petri dish lid. Excess condensation can:
To avoid condensation on your petri dishes, ensure that your growth chamber or room features upward airflow. Upward airflow minimizes the formation of condensation.
For best results, petri dishes should be placed on wire mats on perforated air shelves inside the chamber. Airflow directed upward through these shelves at the underside of the dish ensures the lower zone of the vessel remains cooler than the upper zone. This internal temperature distribution minimizes condensation on the underside of the lid.
Also note that stacking of multiple petri dishes will work against the benefit of upward airflow. See diagram below for a suitable upward airflow design with perforated air shelves and wire mat design:
The following video is an example of a plant growth chamber with uniform vertically directed airflow:
The purpose of this blog is to provide insight into the technologies associated with plant growth chambers around the world by showcasing their usage in universities, institutes and other research centers of excellence.
The blog draws upon the work of clients and other users of controlled environment equipment, Conviron subject matter experts and Conviron’s long history as the world’s leading manufacturer of controlled environments.